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Zea mays Annexins Modulate Cytosolic Free Ca2+ and Generate a Ca2+-Permeable Conductance[W]

机译：玉米Annex膜联蛋白调节细胞内游离钙离子并产生可渗透钙离子的电导[W]

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Regulation of reactive oxygen species and cytosolic free calcium ([Ca2+]cyt) is central to plant function. Annexins are small proteins capable of Ca2+-dependent membrane binding or membrane insertion. They possess structural motifs that could support both peroxidase activity and calcium transport. Here, a Zea mays annexin preparation caused increases in [Ca2+]cyt when added to protoplasts of Arabidopsis thaliana roots expressing aequorin. The pharmacological profile was consistent with annexin activation (at the extracellular plasma membrane face) of Arabidopsis Ca2+-permeable nonselective cation channels. Secreted annexins could therefore modulate Ca2+ influx. As maize annexins occur in the cytosol and plasma membrane, they were incorporated at the intracellular face of lipid bilayers designed to mimic the plasma membrane. Here, they generated an instantaneously activating Ca2+-permeable conductance at mildly acidic pH that was sensitive to verapamil and Gd3+ and had a Ca2+-to-K+ permeability ratio of 0.36. These results suggest that cytosolic annexins create a Ca2+ influx pathway directly, particularly during stress responses involving acidosis. A maize annexin preparation also demonstrated in vitro peroxidase activity that appeared independent of heme association. In conclusion, this study has demonstrated that plant annexins create Ca2+-permeable transport pathways, regulate [Ca2+]cyt, and may function as peroxidases in vitro.
机译：活性氧和胞质游离钙（[Ca2 +] cyt）的调节对植物功能至关重要。膜联蛋白是能够与Ca 2+依赖的膜结合或膜插入的小蛋白。它们具有可以同时支持过氧化物酶活性和钙转运的结构基序。在这里，当添加到表达水母发光蛋白的拟南芥根的原生质体中时，玉米（Zea mays）膜联蛋白制剂会导致[Ca2 +] cyt的增加。药理学特征与拟南芥Ca2 +渗透性非选择性阳离子通道的膜联蛋白活化（在细胞外质膜表面）一致。因此，分泌的膜联蛋白可以调节Ca2 +的流入。当玉米膜联蛋白出现在细胞质和质膜中时，它们被掺入脂质双层的细胞内表面，该脂质双层旨在模拟质膜。在这里，他们在对维拉帕米和Gd3 +敏感的弱酸性pH值上产生了瞬时激活Ca2 +渗透性的电导，并且Ca2 +与K +的渗透性比为0.36。这些结果表明，胞质膜联蛋白直接产生Ca2 +流入途径，特别是在涉及酸中毒的应激反应期间。玉米膜联蛋白制剂还显示了似乎与血红素缔合无关的体外过氧化物酶活性。总而言之，这项研究表明植物膜联蛋白可形成可渗透Ca2 +的转运途径，调节[Ca2 +] cyt，并可能在体外起过氧化物酶的作用。

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